• Certified Lab Equipment & Reagents
  • Fast Dispatch on In-Stock Items
  • Expert Scientific Support

Product details

Cytokeratin, HMW (AE-3), 1mg/mL

Catalog number BNUM0257-50

Price for BNUM0257-50

Sign In for Pricing

Size / count 1x 50 µL

About this product

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1) ; 64 kDa (CK3) ; 59 kDa (CK4) ; 58 kDa (CK5) ; 56 kDa (CK6) ; 52 kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNUM0257-50
Size / count
1x 50 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P) ; Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
KRT76
Gene ID
51350
NCBI Gene ID
654392
UniProt
Q01546
Swiss Prot
Q01546
Host
Mouse
Species Reactivity
Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat
Reactivity
Cytokeratin, HMW
Immunogen
Human epidermal keratin
Target Antigen
Cytokeratin, HMW
Clonality
Monoclonal
Clone
AE-3
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, intracellular|IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |WB (published)
Field of Research
Cancer, Cytoskeleton
Positive Control
Epithelial cells, Skin or Adenocarcinomas
Stability
2 years
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
52-67 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1982) 95: 580-588. (IHC, frozen; IF; WB)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse)
Classification
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Conjugate
Purified, BSA-free

Frequently Asked Questions

What is the catalog number for Cytokeratin, HMW (AE-3), 1mg/mL?

The catalog number for Cytokeratin, HMW (AE-3), 1mg/mL is BNUM0257-50.

What sizes are available for Cytokeratin, HMW (AE-3), 1mg/mL?

Cytokeratin, HMW (AE-3), 1mg/mL is available in the following size(s): 1x 50 µL.

Is Cytokeratin, HMW (AE-3), 1mg/mL in stock?

Cytokeratin, HMW (AE-3), 1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin, HMW (AE-3), 1mg/mL?

Cytokeratin, HMW (AE-3), 1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin, HMW (AE-3), 1mg/mL?

Estimated delivery time for Cytokeratin, HMW (AE-3), 1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin, HMW (AE-3), 1mg/mL?

The safety data sheet for Cytokeratin, HMW (AE-3), 1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin, HMW (AE-3), 1mg/mL used for?

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1) ; 64 kDa (CK3) ; 59 kDa (CK4) ; 58 kDa (CK5) ; 56 kDa (CK6) ; 52 kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have mole

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

You Might Also Like
Mucin 5AC (MUC5AC) (58M1), CF405S conjugate, 0.1mg/mL
Mucin 5AC (MUC5AC) (58M1), CF405S conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC041003-500
CD11c (HC1/1), CF680R conjugate, 0.1mg/mL
CD11c (HC1/1), CF680R conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC810152-500
CD47 (B6H12.2), CF568 conjugate, 0.1mg/mL
CD47 (B6H12.2), CF568 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC680437-100
HSP27 (G3.1), CF647 conjugate, 0.1mg/mL
HSP27 (G3.1), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC470861-100
CD45RO (190-2F2.5), CF405S conjugate, 0.1mg/mL
CD45RO (190-2F2.5), CF405S conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC041081-500
Major Vault Protein (MVP) (1032), CF488A conjugate, 0.1mg/mL
Major Vault Protein (MVP) (1032), CF488A conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC880225-100
Cytochrome C (CTC05), CF740 conjugate, 0.1mg/mL
Cytochrome C (CTC05), CF740 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC740887-500
CD8A (Cytotoxic- & Suppressor T-Cell Marker) (C8/1779R), CF405S conjugate, 0.1mg/mL
CD8A (Cytotoxic- & Suppressor T-Cell Marker) (C8/1779R), CF405S conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC041779-500