Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL

Catalog number BNCB0257-100

Price for BNCB0257-100

Size / count 1x 100 µL

About this product

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1) ; 64 kDa (CK3) ; 59 kDa (CK4) ; 58 kDa (CK5) ; 56 kDa (CK6) ; 52 kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB0257-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P) ; Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT76
Gene ID: 51350
NCBI Gene ID: 654392
UniProt: Q01546
Swiss Prot: Q01546
Host: Mouse
Species Reactivity: Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat
Reactivity: Cytokeratin, HMW
Immunogen: Human epidermal keratin
Target Antigen: Cytokeratin, HMW
Clonality: Monoclonal
Clone: AE-3
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular|IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |WB (published)
Field of Research: Cancer, Cytoskeleton
Positive Control: Epithelial cells, Skin or Adenocarcinomas
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 52-67 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1982) 95: 580-588. (IHC, frozen; IF; WB)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL?

The catalog number for Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL is BNCB0257-100.

What sizes are available for Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL?

Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL in stock?

Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL?

Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL?

The safety data sheet for Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin, HMW (AE-3), Biotin conjugate, 0.1mg/mL used for?

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.