Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL

Catalog number BNC040257-500

Price for BNC040257-500

Size / count 1x 500 µL

About this product

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1) ; 64 kDa (CK3) ; 59 kDa (CK4) ; 58 kDa (CK5) ; 56 kDa (CK6) ; 52 kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC040257-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P) ; Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT76
Gene ID: 51350
NCBI Gene ID: 654392
UniProt: Q01546
Swiss Prot: Q01546
Host: Mouse
Species Reactivity: Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat
Reactivity: Cytokeratin, HMW
Immunogen: Human epidermal keratin
Target Antigen: Cytokeratin, HMW
Clonality: Monoclonal
Clone: AE-3
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular|IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |WB (published)
Field of Research: Cancer, Cytoskeleton
Positive Control: Epithelial cells, Skin or Adenocarcinomas
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 52-67 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1982) 95: 580-588. (IHC, frozen; IF; WB)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF405S

Frequently Asked Questions

What is the catalog number for Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL?

The catalog number for Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL is BNC040257-500.

What sizes are available for Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL?

Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL in stock?

Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL?

Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL?

Estimated delivery time for Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL?

The safety data sheet for Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin, HMW (AE-3), CF405S conjugate, 0.1mg/mL used for?

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.