Cytokeratin, LMW (AE-1), CF488A conjugate, 0.1mg/mL

About this product

This MAb recognizes the 56.5 kDa (CK10) ; 50 kDa (CK14) ; 50 kDa (CK15) ; 48 kDa (CK16) ; 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC880253-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT77

Gene ID

374454

NCBI Gene ID

334989

UniProt

Q7Z794

Swiss Prot

Q7Z794

Host

Mouse

Species Reactivity

Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat, Turtle

Reactivity

Cytokeratin, LMW

Immunogen

Human epidermal keratin

Target Antigen

Cytokeratin, LMW

Clonality

Monoclonal

Clone

AE-1

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published) |IHC, FFPE (verified) |WB (verified)

Field of Research

Cancer, Cytoskeleton

Positive Control

Skin, Squamous cell carcinoma (SCC)

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

40-56.5 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Ann N Y Acad Sci (1986) 468: 302-15. (Flow)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF488A