Eosinophil Peroxidase (AHE-1), CF594 conjugate, 0.1mg/mL

About this product

Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO) . Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC941231-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX; EPX PEN; PERE

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

EPX

Gene ID

8288

NCBI Gene ID

279259

UniProt

P11678

Swiss Prot

P11678

Host

Mouse

Species Reactivity

Human

Reactivity

Eosinophil Peroxidase

Immunogen

Human eosinophils from a patient with hypereosinophilic syndrome

Target Antigen

Eosinophil Peroxidase

Clonality

Monoclonal

Clone

AHE-1

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (verified)

Field of Research

Immunology

Positive Control

MCF-7 cells. Tonsil

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

70 kDa (55 kDa-Heavy Chain; 15 kDa-Light Chain)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Leukocyte Biol (1989) 46:109-118. (RIA) J Exp Med (1991) 173, 117-126. (IHC, FFPE) Clin Exp Immunol (1993) 92:58-64. (ELISA, IP, functional studies, Flow) Gastroenterology (2011) 142(1): 15-20. (IHC, frozen)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF594