Ep-CAM / CD326 (HEA125), 1mg/mL

About this product

Note: All CF® Dye and biotin conjugates of this antibody have been discontinued. The antibody is still available unlabeled, with or without BSA. This antibody recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM0825-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Adenocarcinoma-associated Antigen | Cell Surface Glycoprotein Trop-1 | Chromosome 17 | ECS-1 | EGP2 | EGP314 | EGP40 | Epidermal Surface Antigen 1 | Epithelial Cell Adhesion Molecule | Epithelial Glycoprotein 314 | ESA | ESA1 | FLOT2 | Flotillin-2 | KSA | Membrane Component | REG-1 | Reggie-1 | Reggie-2 | Surface Marker-1 (M17S1) | TACD1 | TROP1 | Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TACSTD1

Gene ID

4072

NCBI Gene ID

542050

UniProt

P16422

Swiss Prot

P16422

Host

Mouse

Species Reactivity

Human

Reactivity

CD326|Ep-CAM

Immunogen

Recombinant human EpCAM protein fragment from the extracellular domain (aa77-202) (exact sequence is proprietary)

Target Antigen

CD326 | Ep-CAM

Clonality

Monoclonal

Clone

HEA125

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, frozen (published) |Flow, surface (verified) |IF (verified) |IHC, FFPE (verified) |IP (published) |RIA (published)

Field of Research

Cancer, Cell adhesion, Exosomes/EVs

Positive Control

HT29 cells or Ovarian carcinoma

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

40-43 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Br J Cancer (1987) 56: 714-721. (immunoprecipitation; RIA; IHC, frozen)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free