Fascin-1 (FSCN1/418), 0.2mg/mL

About this product

Recognizes a protein of 55 kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0418-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

55kDa actin-bundling protein; FAN1; Fascin homolog 1; Fascin1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like) ; SNL

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FSCN1

Gene ID

6624

NCBI Gene ID

118400

UniProt

Q16658

Swiss Prot

Q16658

Host

Mouse

Species Reactivity

Human, Rat

Reactivity

Fascin-1

Immunogen

Full length recombinant human FSCN1 protein

Target Antigen

Fascin-1

Clonality

Monoclonal

Clone

FSCN1/418

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)

Field of Research

Cancer, Immunology

Positive Control

HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

55 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA