Fibronectin (HFN7.1), 0.2mg/mL

About this product

Fibronectin is a dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0270-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Cold insoluble globulin (CIG) ; FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF) ; Ugl-Y3

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FN1

Gene ID

2335

NCBI Gene ID

203717

UniProt

P02751

Swiss Prot

P02751

Host

Mouse

Species Reactivity

Human

Reactivity

Fibronectin

Immunogen

Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose

Target Antigen

Fibronectin

Clonality

Monoclonal

Clone

HFN7.1

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published) |IF (published) |ELISA (published) |IHC, FFPE (verified) |WB (published)

Field of Research

Cell adhesion, Extracellular matrix

Positive Control

SW156 cells or Kidney

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

220 kDa (monomer) ; 440 kDa (dimer)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1991) 266(14): 23323-23328. (inhibition of integrin binding; epitope mapping) Anat Rec (1996) 245:068-676. (IF, frozen tissue) PNAS USA (2005) 102(17): 5953-5957. (functional studies) Biomaterials (2005) 26: 4523-4531. (IF; functional studies) Hybridoma (2006) 25(4): 202-208. (ELISA; WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA