FOXP3 (3G3), Biotin conjugate, 0.1mg/mL

About this product

Recognizes a protein of 47-55 kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion (amino acids 2-136) of the protein.The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB0645-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

JM2, AIID, DIETER, IPEX, MGC141961, MGC141963, PIDX, XPID, FOXP3delta7, forkhead box protein P3, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, scurfin

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FOXP3

Gene ID

50943

NCBI Gene ID

247700

UniProt

Q9BZS1

Swiss Prot

Q9BZS1

Host

Mouse

Species Reactivity

Human, Monkey, Mouse

Reactivity

FOXP3

Immunogen

Full-length His-tagged mouse FOXP3 protein

Target Antigen

FOXP3

Clonality

Monoclonal

Clone

3G3

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published)

Field of Research

Cancer, Developmental biology, Endocrinology, Transcription factors

Positive Control

Tonsil, lymph node or Breast carcinoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

47-55 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (2006) 103(17): 6659-6664. (Flow; epitope mapping) Cytometry Part A (2009) 75A: 1040-1050. (Flow; comparison of anti-FOXP3 clones and fixation methods)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin