CD7 (T3-3A1), CF740 conjugate, 0.1mg/mL

About this product

Recognizes a protein of 40 kDa, identified as CD7, a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC740978-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD27 ligand (CD27L) ; CD27LG; Ki24; Surface antigen CD70; Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD7

Gene ID

924

NCBI Gene ID

186820

UniProt

P09564

Swiss Prot

P09564

Host

Mouse

Species Reactivity

Human

Reactivity

CD7

Immunogen

Human T cells

Target Antigen

CD7

Clonality

Monoclonal

Clone

T3-3A1

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |Functional studies (published) |IF (published) |ELISA (published) |IP (published)

Field of Research

Immunology, Signal transduction

Positive Control

Jurkat, HUT-78, Molt-4, CEM cells, or human peripheral blood lymphocytes. Lymph node and tonsil.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

40 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1979) 76: 5829-5833. PNAS USA (1980) 77(5): 2914-2918. (IP; functional studies) J Immunol Meth (1987) 96(2), 201-209. (cell ELISA) PNAS USA (1996) 93: 10348-10353. (cell depletion)>/li> Blood (1998) 91(8) 2760-2771. (Flow) J Cell Biol (1998) 142(5): 1245-1256. (IF; IP) Theranostics (2018) 8(21): 6070-6087. (mAb characterization; in vivo imaging)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), Bos taurus (bovine)

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF740