Golgi Complex (371-4), Biotin conjugate, 0.1mg/mL

About this product

This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB0102-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Golgi Complex; Golgi apparatus; Golgi zone

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

Not Known

NCBI Gene ID

Not Known

UniProt

Not Known

Swiss Prot

Not Known

Host

Mouse

Species Reactivity

Human

Reactivity

Golgi Complex

Immunogen

SU-DHL-1 large cell lymphoma cells.

Target Antigen

Golgi Complex

Clonality

Monoclonal

Clone

371-4

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |IHC, FFPE (verified)

Field of Research

Organelle markers

Positive Control

HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

Not Known

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (2001) 61: 3339-3347. (IF) J Virol (2005) 79(13): 8506. (IF)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin