HLA-Aw32 & HLA-A25 (MHC I) (CATA-1), CF647 conjugate, 0.1mg/mL

About this product

This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC471073-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

HLA-A; HLA class 1A; MHC class I HLA-A

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

HLA-A

Gene ID

3105

NCBI Gene ID

181244; 654404; 77961

UniProt

P01889; P30443; P30499

Swiss Prot

P01889; P30443; P30499

Host

Mouse

Species Reactivity

Human

Reactivity

HLA-Aw32-&-HLA-A25

Immunogen

Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10

Target Antigen

HLA-Aw32-&-HLA-A25

Clonality

Monoclonal

Clone

CATA-1

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, frozen (published) |IHC, FFPE (verified)

Field of Research

Cancer, Immunology, MHC antigens

Positive Control

THP-1 and Jurkat cells. Tonsil or lymph node.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

KDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Tissue Antigens (2004) 64: 696-702. (IHC, frozen) Clin Cancer Res (2005) 11: 498-504. (IHC, frozen)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647