Product details

HSP60 (LK1), 0.2mg/mL

Catalog number BNUB0851-100

Price for BNUB0851-100

Size / count 1x 100 µL

About this product

Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60) . Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSPs), more appropriately called as stress response proteins (SRPs) . hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB0851-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: 60kDa chaperonin; 60kDa heat shock protein mitochondrial; Chaperonin; 60-KD (CPN60) ; GROEL; HLD4; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (SPG13)
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: HSPD1
Gene ID: 3329
NCBI Gene ID: 595053
UniProt: P10809
Swiss Prot: P10809
Host: Mouse
Species Reactivity: Chicken, Cow, Dog, Drosophila, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus
Reactivity: HSP60
Immunogen: Recombinant human HSP60 protein
Target Antigen: HSP60
Clonality: Monoclonal
Clone: LK1
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |IP (published) |WB (verified)
Field of Research: Autoimmunity, Heat shock proteins, Immunology, Inflammation
Positive Control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 60 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Lett (2017) 385: 75-86. (immunoprecipitation)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for HSP60 (LK1), 0.2mg/mL?

The catalog number for HSP60 (LK1), 0.2mg/mL is BNUB0851-100.

What sizes are available for HSP60 (LK1), 0.2mg/mL?

HSP60 (LK1), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is HSP60 (LK1), 0.2mg/mL in stock?

HSP60 (LK1), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures HSP60 (LK1), 0.2mg/mL?

HSP60 (LK1), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for HSP60 (LK1), 0.2mg/mL?

Estimated delivery time for HSP60 (LK1), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for HSP60 (LK1), 0.2mg/mL?

The safety data sheet for HSP60 (LK1), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is HSP60 (LK1), 0.2mg/mL used for?

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.