HSP60 (LK1), CF405S conjugate, 0.1mg/mL

About this product

Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60) . Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSPs), more appropriately called as stress response proteins (SRPs) . hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC040851-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

60kDa chaperonin; 60kDa heat shock protein mitochondrial; Chaperonin; 60-KD (CPN60) ; GROEL; HLD4; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (SPG13)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

HSPD1

Gene ID

3329

NCBI Gene ID

595053

UniProt

P10809

Swiss Prot

P10809

Host

Mouse

Species Reactivity

Chicken, Cow, Dog, Drosophila, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus

Reactivity

HSP60

Immunogen

Recombinant human HSP60 protein

Target Antigen

HSP60

Clonality

Monoclonal

Clone

LK1

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |IP (published) |WB (verified)

Field of Research

Autoimmunity, Heat shock proteins, Immunology, Inflammation

Positive Control

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

60 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Lett (2017) 385: 75-86. (immunoprecipitation)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S