Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL

Catalog number BNC400827-500

Price for BNC400827-500

Size / count 1x 500 µL

About this product

This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC400827-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: IVL
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: IVL
Gene ID: 3713
NCBI Gene ID: 516439
UniProt: P07476
Swiss Prot: P07476
Host: Mouse
Species Reactivity: Human
Reactivity: Involucrin
Immunogen: Purified involucrin from human keratinocytes
Target Antigen: Involucrin
Clonality: Monoclonal
Clone: IVRN/827
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Field of Research: Cancer
Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 66-170 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF640R

Frequently Asked Questions

What is the catalog number for Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL?

The catalog number for Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL is BNC400827-500.

What sizes are available for Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL?

Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL in stock?

Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL?

Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL?

Estimated delivery time for Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL?

The safety data sheet for Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Involucrin (IVRN/827), CF640R conjugate, 0.1mg/mL used for?

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.