Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL — image 1 of 4

Product details

Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL

Catalog number BNC470729-500

Price for BNC470729-500

Size / count 1x 500 µL

About this product

Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC470729-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Ku (p70) : 70kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70kDa subunit; CTC box-binding factor 75kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen, 70kDa; Ku70; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen) ; Thyroid-lupus autoantigen (TLAA) ; X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80) : 86kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86Kd subunit; ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV) ; Thyroid-lupus autoantigen (TLAA) ; X-ray repair cross-complementing protein 5 (XRCC5)
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: XRCC6
Gene ID: 2547 (p70); 7520 (p80)
NCBI Gene ID: 292493 (p70); 388739 (p80)
UniProt: P12956 (p70); P13010 (p80)
Swiss Prot: P12956 (p70) ; P13010 (p80)
Host: Mouse
Species Reactivity: Human, Non-human primates
Reactivity: Ku-Holo
Immunogen: Nuclear extract of human HL-60 cells
Target Antigen: Ku-Holo
Clonality: Monoclonal
Clone: KU729
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified)
Field of Research: Apoptosis, Cell cycle, Signal transduction
Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 70 kDa & 80 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF647

Frequently Asked Questions

What is the catalog number for Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL?

The catalog number for Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL is BNC470729-500.

What sizes are available for Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL?

Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL in stock?

Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL?

Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL?

Estimated delivery time for Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL?

The safety data sheet for Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02ae061270361e108c50/msds/bnc470729-500.pdf.

What is Ku-Holo (p70;83) (KU729), CF647 conjugate, 0.1mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.