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Product details

Laminin (A5), CF647 conjugate, 0.1mg/mL

Catalog number BNC470308-100

Price for BNC470308-100

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Size / count 1x 100 µL

About this product

Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of α , β , and γ chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM) . This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC470308-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
LAMB2; LAMC1; Laminin B2 chain; Laminin gamma 1; Laminin gamma 1 chain
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
LAMC1
Gene ID
3915
NCBI Gene ID
609663
UniProt
P11047, Q5VYE7; Q6NVY8
Swiss Prot
P11047, Q5VYE7 & Q6NVY8
Host
Rat
Species Reactivity
Human, Mouse
Reactivity
Laminin
Immunogen
Murine EHS laminin preparation
Target Antigen
Laminin
Clonality
Monoclonal
Clone
A5
Type
Primary
Source
Animal
Origin
Animal
Field of Research
Cancer
Positive Control
Normal colon or carcinomas
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
210 kDa
References & Citations
Ljubimov AV et. al., Int J Cancer, 1992; 50:562-566. | Ljubimov AV et. al. Lab Investigation, 1995; 72:461-473. | Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Rattus norvegicus (laboratory rat), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF647

Frequently Asked Questions

What is the catalog number for Laminin (A5), CF647 conjugate, 0.1mg/mL?

The catalog number for Laminin (A5), CF647 conjugate, 0.1mg/mL is BNC470308-100.

What sizes are available for Laminin (A5), CF647 conjugate, 0.1mg/mL?

Laminin (A5), CF647 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Laminin (A5), CF647 conjugate, 0.1mg/mL in stock?

Laminin (A5), CF647 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Laminin (A5), CF647 conjugate, 0.1mg/mL?

Laminin (A5), CF647 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Laminin (A5), CF647 conjugate, 0.1mg/mL?

Estimated delivery time for Laminin (A5), CF647 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Laminin (A5), CF647 conjugate, 0.1mg/mL?

The safety data sheet for Laminin (A5), CF647 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02b5061270361e10b315/msds/bnc470308-100.pdf.

What is Laminin (A5), CF647 conjugate, 0.1mg/mL used for?

Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of α , β , and γ chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipita

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

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