Lewis A (7LE), Biotin conjugate, 0.1mg/mL

About this product

Recognizes a carbohydrate determinant of Gal 1-3 (Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB0311-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Lewis A antigen

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

UniProt

Not Applicable

Swiss Prot

Not Applicable

Host

Mouse

Species Reactivity

Human, Mouse

Reactivity

Lewis-A

Immunogen

Mucins isolated from ovarian cyst fluid

Target Antigen

Lewis-A

Clonality

Monoclonal

Clone

7LE

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |ELISA (published) |IHC, FFPE (verified)

Field of Research

Cancer

Positive Control

Colon tissue

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

Multiple

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (2002) 62: 4194-4198. (ELISA) Biol Pharm Bull (1999) 22(4):347-52. (Flow, surface)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin