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Product details

Lewis A (7LE), CF488A conjugate, 0.1mg/mL

Catalog number BNC880311-100

Price for BNC880311-100

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Size / count 1x 100 µL

About this product

Recognizes a carbohydrate determinant of Gal 1-3 (Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC880311-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Lewis A antigen
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
UniProt
Not Applicable
Swiss Prot
Not Applicable
Host
Mouse
Species Reactivity
Human, Mouse
Reactivity
Lewis-A
Immunogen
Mucins isolated from ovarian cyst fluid
Target Antigen
Lewis-A
Clonality
Monoclonal
Clone
7LE
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, surface (published) |ELISA (published) |IHC, FFPE (verified)
Field of Research
Cancer
Positive Control
Colon tissue
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
Multiple
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (2002) 62: 4194-4198. (ELISA) Biol Pharm Bull (1999) 22(4):347-52. (Flow, surface)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF488A

Frequently Asked Questions

What is the catalog number for Lewis A (7LE), CF488A conjugate, 0.1mg/mL?

The catalog number for Lewis A (7LE), CF488A conjugate, 0.1mg/mL is BNC880311-100.

What sizes are available for Lewis A (7LE), CF488A conjugate, 0.1mg/mL?

Lewis A (7LE), CF488A conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Lewis A (7LE), CF488A conjugate, 0.1mg/mL in stock?

Lewis A (7LE), CF488A conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Lewis A (7LE), CF488A conjugate, 0.1mg/mL?

Lewis A (7LE), CF488A conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Lewis A (7LE), CF488A conjugate, 0.1mg/mL?

Estimated delivery time for Lewis A (7LE), CF488A conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Lewis A (7LE), CF488A conjugate, 0.1mg/mL?

The safety data sheet for Lewis A (7LE), CF488A conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Lewis A (7LE), CF488A conjugate, 0.1mg/mL used for?

Recognizes a carbohydrate determinant of Gal 1-3 (Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

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