MART-1 / Melan-A (M2-7C10), CF488A conjugate, 0.1mg/mL

About this product

This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC880009-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

MLANA

Gene ID

2315

NCBI Gene ID

154069

UniProt

Q16655

Swiss Prot

Q16655

Host

Mouse

Species Reactivity

Horse, Human

Reactivity

MART-1|Melan-A

Immunogen

Recombinant hMART-1 protein

Target Antigen

MART-1 | Melan-A

Clonality

Monoclonal

Clone

M2-7C10

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published) |IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |WB (published)

Field of Research

Cancer

Positive Control

SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

20-22 kDa (doublet)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Int J Cancer (1998) 75, 517-524. (IHC, frozen; IHC, cytospin) Cancer Cytopathol (1999) 87(1): 37-42. (IHC, FFPE; IHC, cytospin) Mol Med Rep (2011) 4: 799-803. (WB; IF; IHC) World J Immunol (2013) 3(3): 62-67. (Flow, intracellular)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF488A