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Product details

Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL

Catalog number BNC040702-500

Price for BNC040702-500

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Size / count 1x 500 µL

About this product

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC040702-500
Size / count
1x 500 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene ID
2315-MART-1; 7299-Tyrosinase; 6490-gp100
NCBI Gene ID
154069-MART-1; 503555-Tyrosinase; 95972-gp100
UniProt
Q16655-MART-1; P14679-Tyrosinase; P40967-gp100
Swiss Prot
Q16655-MART-1; P14679-Tyrosinase; P40967-gp100
Host
Mouse
Species Reactivity
Human
Reactivity
Melanoma Marker
Immunogen
Recombinant hMART-1 protein (A103) ; Recombinant tyrosinase protein (T311) ; Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Target Antigen
Melanoma Marker
Clonality
Monoclonal
Clone
HMB45 A103 T311
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified) |WB (verified)
Field of Research
Cancer
Positive Control
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
20-22 kDa (doublet) (MART) ; 70-80 kDa (Tyrosinase) ; 90-100 kDa (gp100)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG' s
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF405S

Frequently Asked Questions

What is the catalog number for Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL?

The catalog number for Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL is BNC040702-500.

What sizes are available for Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL?

Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL in stock?

Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL?

Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL?

Estimated delivery time for Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL?

The safety data sheet for Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Melanoma Marker (HMB45 + A103 + T311), CF405S conjugate, 0.1mg/mL used for?

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melan

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

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