Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL

Catalog number BNC940702-100

Price for BNC940702-100

Size / count 1x 100 µL

About this product

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC940702-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene ID: 2315-MART-1; 7299-Tyrosinase; 6490-gp100
NCBI Gene ID: 154069-MART-1; 503555-Tyrosinase; 95972-gp100
UniProt: Q16655-MART-1; P14679-Tyrosinase; P40967-gp100
Swiss Prot: Q16655-MART-1; P14679-Tyrosinase; P40967-gp100
Host: Mouse
Species Reactivity: Human
Reactivity: Melanoma Marker
Immunogen: Recombinant hMART-1 protein (A103) ; Recombinant tyrosinase protein (T311) ; Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Target Antigen: Melanoma Marker
Clonality: Monoclonal
Clone: HMB45 A103 T311
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Field of Research: Cancer
Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 20-22 kDa (doublet) (MART) ; 70-80 kDa (Tyrosinase) ; 90-100 kDa (gp100)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG' s
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL?

The catalog number for Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL is BNC940702-100.

What sizes are available for Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL?

Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL in stock?

Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL?

Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL?

The safety data sheet for Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Melanoma Marker (HMB45 + A103 + T311), CF594 conjugate, 0.1mg/mL used for?

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.