MHC II (HLA-DQ) (SPV-L3), CF647 conjugate, 0.1mg/mL

About this product

Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages) . The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470200-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

3117; 3118; 3119

NCBI Gene ID

550475

UniProt

P01908; P01909; P01920

Swiss Prot

P01908; P01909; P01920

Host

Mouse

Species Reactivity

Human, Pig

Reactivity

MHC-II

Immunogen

T4-positive CTL clone HG-38

Target Antigen

MHC-II

Clonality

Monoclonal

Clone

SPV-L3

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published) |Flow, surface (verified) |IF (verified) |IP (published) |WB (published)

Field of Research

Immunology, MHC antigens

Positive Control

Raji cells. Tonsil or lymph node

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

60 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Hybridoma (1983) 2(4):423-37. (functional studies) Eur J Immunol (1984) 14: 299-304. (immunoprecipitation) J Biol Chem (2010) 285(52): 40800-40808. (western)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647