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Product details

Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL

Catalog number BNUB0743-100

Price for BNUB0743-100

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Size / count 1x 100 µL

About this product

In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. The α subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The β subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNUB0743-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Breast carcinoma-associated antigen DF3; CA15-3; Carcinoma-associated mucin Episialin; Epithelial Membrane Antigen; H23AG; KL-6; MAM6; MUC-1; MUC-1/SEC; MUC-1/X; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; Mucin 1 cell surface associated; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
MUC1
Gene ID
4582
NCBI Gene ID
89603
UniProt
P15941
Swiss Prot
P15941
Host
Mouse
Species Reactivity
Human
Reactivity
CD227|EMA|Episialin|Mucin 1
Immunogen
Human milk fat globule membranes (GP1.4) ; Delipidated extract of human milk fat globule membranes (E29)
Target Antigen
CD227 | EMA | Episialin | Mucin 1
Clonality
Monoclonal
Clone
GP1.4 E29
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified)
Field of Research
Cancer, Mucins
Positive Control
MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
265-400 kDa
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG' s
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA

Frequently Asked Questions

What is the catalog number for Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL?

The catalog number for Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL is BNUB0743-100.

What sizes are available for Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL?

Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL in stock?

Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL?

Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL?

Estimated delivery time for Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL?

The safety data sheet for Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Mucin 1 / EMA / Episialin / CD227 (GP1.4 + E29), 0.2mg/mL used for?

In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. The α subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial a

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

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