Mucin 5AC (MUC5AC) (45M1), CF647 conjugate, 0.1mg/mL

About this product

This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC) . Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. The epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470031-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

MUC5AC

Gene ID

4586

NCBI Gene ID

534332

UniProt

P98088

Swiss Prot

P98088

Host

Mouse

Species Reactivity

Cat, Chicken, Hedgehog, Human, Monkey, Mouse, Pig, Rabbit, Rat

Reactivity

Mucin 5AC

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient

Target Antigen

Mucin 5AC

Clonality

Monoclonal

Clone

45M1

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |ELISA (published) |IHC, FFPE (verified) |WB (published)

Field of Research

Cancer, Mucins

Positive Control

Stomach

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

>1,000 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Biochem J (2002) 364, 191-200. (IHC, FFPE; ELISA) FEBS Journal 275 (2008): 481-489. (WB; epitope mapping) Cancer Immunol Immunother (2013) 62: 1011-1019. (Flow, surface)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647