EEA1 Recombinant Rabbit Monoclonal Antibody [JE59-34]

About this product

The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. EEA1 localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms a homodimer through a coiled coil. EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. EEA1 is a RAB5A effector protein which binds via an N-terminal zinc finger domain and is required for fusion of early and late endosomes and for sorting at the early endosome level. EEA1 plays a role in endocytosis and is recruited by Rab5-GTP to endosomal membranes. EEA1 may be regulated through monoubiquination, affecting endosome fusion and trafficking. Ubiquitin selective segregase p97 may regulate EEA1's tethering ability, affecting its endosome trafficking and morphplogy.

Specifications

Catalog number

HA722147

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Early endosome antigen 1 antibody Early endosome antigen 1, 162kD antibody Early endosome associated protein antibody EEA 1 antibody EEA1 antibody EEA1_HUMAN antibody Endosome associated protein p162 antibody Endosome-associated protein p162 antibody MST105 antibody MSTP105 antibody ZFYVE2 antibody Zinc finger FYVE domain containing protein 2 antibody Zinc finger FYVE domain-containing protein 2 antibody

Abbreviation

EEA1, ZFYVE2

Swiss Prot

Q15075 Human, Q8BL66 Mouse,

Cellular Locus

Cytoplasm, Early endosome membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within Human EEA1 aa 1-100 / 1,411.

Isotype

IgG

Clone

JE59-34

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, FC

Positive Control

HeLa cell lysate, Jurkat cell lysate, JAR cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, JAR, NIH/3T3, C6.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 162 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes