Neurofilament (H+L) (RT-97 + NR-4), CF640R conjugate, 0.1mg/mL

About this product

This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L) . Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H) . Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC401201-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

NEFH

Gene ID

4744

NCBI Gene ID

198760

UniProt

P12036

Swiss Prot

P12036

Host

Mouse

Species Reactivity

Chicken, Human, Mouse, Pig, Rat

Reactivity

Neurofilament

Immunogen

Triton-X 100 insoluble proteins of rat brain (RT-97) ; Neurofilaments from porcine spinal cord (NR-4)

Target Antigen

Neurofilament

Clonality

Monoclonal

Clone

RT-97 NR-4

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (verified) |IHC, FFPE (verified)

Field of Research

Cancer, Cytoskeleton, Neuroscience

Positive Control

Cerebellum, Neuroblastoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

200 kDa & 68 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG' s

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF640R