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Product details

Nucleolin (364-5), 1mg/mL

Catalog number BNUM0527-50

Price for BNUM0527-50

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Size / count 1x 50 µL

About this product

Recognizes Nucleolin (NCL), the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11 kb. It has a predicted molecular weight of ~76 kDa with an apparent MW of 100-110 kDa, attributed to phosphorylation of its N-terminal domain. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde- or methanol-fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNUM0527-50
Size / count
1x 50 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
NCL; Nucl; Nucleolin; Protein C23
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
NCL
Gene ID
4691
NCBI Gene ID
79110
UniProt
P19338
Swiss Prot
P19338
Host
Mouse
Species Reactivity
Human
Reactivity
Nucleolin
Immunogen
Lysate of SU-DHL-1 Nuclei
Target Antigen
Nucleolin
Clonality
Monoclonal
Clone
364-5
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, intracellular (verified) |IF (verified) |IHC, FFPE (verified) |WB (verified)
Field of Research
Organelle markers
Positive Control
All human cells
Stability
2 years
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
76 kDa (predicted), 100-110 kDa (apparent)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse)
Classification
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Conjugate
Purified, BSA-free

Frequently Asked Questions

What is the catalog number for Nucleolin (364-5), 1mg/mL?

The catalog number for Nucleolin (364-5), 1mg/mL is BNUM0527-50.

What sizes are available for Nucleolin (364-5), 1mg/mL?

Nucleolin (364-5), 1mg/mL is available in the following size(s): 1x 50 µL.

Is Nucleolin (364-5), 1mg/mL in stock?

Nucleolin (364-5), 1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Nucleolin (364-5), 1mg/mL?

Nucleolin (364-5), 1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Nucleolin (364-5), 1mg/mL?

Estimated delivery time for Nucleolin (364-5), 1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Nucleolin (364-5), 1mg/mL?

The safety data sheet for Nucleolin (364-5), 1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd029c061270361e1022dc/msds/bnum0527-50.pdf.

What is Nucleolin (364-5), 1mg/mL used for?

Recognizes Nucleolin (NCL), the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spa

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

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