P21 / WAF1 (WA-1), Biotin conjugate, 0.1mg/mL

About this product

This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma) . In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB0043-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Activating Fragment 1; CAP20; CDK-interacting protein 1; CDKI; CDKN1; CDKN1A; CIP1; Cyclin-dependent kinase inhibitor 1A (p21; Cip1) ; DNA Synthesis Inhibitor; MDA6; Melanoma Differentiation Associated Protein 6; p21Cip1/Waf1; PIC1; SDI1; SLC12A9; Wild type p53 activated fragment 1 (WAF1)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CDKN1A

Gene ID

1026

NCBI Gene ID

370771

UniProt

P38936

Swiss Prot

P38936

Host

Mouse

Species Reactivity

Chimpanzee, Human, Monkey, Mouse, Rat

Reactivity

P21|WAF1

Immunogen

Human recombinant p21 protein

Target Antigen

p21 | WAF1

Clonality

Monoclonal

Clone

WA-1

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published) |IF (published) |IHC, FFPE (verified) |WB (published)

Field of Research

Cell cycle, Tumor suppressors

Positive Control

HeLa Cells. Skin, colon, or breast carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

21 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (2001) 276(14): 11143-11150. (Flow, intracellular; IF) EMBO J (2002) 21:6771-6780. (WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin