P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL

Catalog number BNUB1237-100

Price for BNUB1237-100

Size / count 1x 100 µL

About this product

Recognizes a 27 kDa protein, identified as the p27/Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27/Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-beta-induced G1 arrest. p27/Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27/Kip1 and is excellent for staining of formalin-fixed tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB1237-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CDKN1B
Gene ID: 1027
NCBI Gene ID: 238990
UniProt: P46527
Swiss Prot: P46527
Host: Mouse
Species Reactivity: Human, Monkey, Mouse, Rat
Reactivity: KIP1|p27
Immunogen: Mouse recombinant p27 protein (DCS-72.F6) ; Recombinant human CDKN1B protein (KIP1/769)
Target Antigen: KIP1 | p27
Clonality: Monoclonal
Clone: DCS-72.F6 KIP1/769
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Field of Research: Cell cycle, Tumor suppressors
Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 25-26 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG' s
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL?

The catalog number for P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL is BNUB1237-100.

What sizes are available for P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL?

P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL is available in the following size(s): 1x 100 µL.

Is P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL in stock?

P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL?

P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL?

Estimated delivery time for P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL?

The safety data sheet for P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is P27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8) ), 0.2mg/mL used for?

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.