P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL — image 1 of 3

Product details

P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL

Catalog number BNC470771-500

Price for BNC470771-500

Size / count 1x 500 µL

About this product

Recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-betanduced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC470771-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CDKN1B
Gene ID: 1027
NCBI Gene ID: 238990
UniProt: P46527
Swiss Prot: P46527
Host: Mouse
Species Reactivity: Human, Monkey, Mouse, Rat
Reactivity: KIP1|p27
Immunogen: Mouse recombinant p27 protein
Target Antigen: KIP1 | p27
Clonality: Monoclonal
Clone: DCS-72.F6
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IHC, FFPE (verified)
Field of Research: Cell cycle, Tumor suppressors
Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 25-26 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF647

Frequently Asked Questions

What is the catalog number for P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL?

The catalog number for P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL is BNC470771-500.

What sizes are available for P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL?

P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL in stock?

P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL?

P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL?

Estimated delivery time for P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL?

The safety data sheet for P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02a9061270361e106ea7/msds/bnc470771-500.pdf.

What is P27 / KIP1 (DCS-72.F6), CF647 conjugate, 0.1mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.