P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL — image 1 of 4

Product details

P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL

Catalog number BNC040669-500

Price for BNC040669-500

Size / count 1x 500 µL

About this product

This MAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-betanduced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC040669-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CDKN1B
Gene ID: 1027
NCBI Gene ID: 238990
UniProt: P46527
Swiss Prot: P46527
Host: Mouse
Species Reactivity: Human, Monkey, Mouse, Rat
Reactivity: KIP1|p27
Immunogen: Purified GST-p27 fusion protein of human origin
Target Antigen: KIP1 | p27
Clonality: Monoclonal
Clone: SX53G8
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE|Flow, intracellular (verified) |IF (verified) |IP (published) |WB (published)
Field of Research: Cell cycle, Tumor suppressors
Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 25-26 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications._x000D_ 1. PNAS USA (1997) 94:6380-6385. (western; immunoprecipitation; IHC, FFPE)_x000D_ 2. Blood (2005) 105(9): 3691. (western)_x000D_ 3. Anticancer Res (2017) 37: 2407-2415. (IHC, FFPE; IF, western)_x000D_
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF405S

Frequently Asked Questions

What is the catalog number for P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL?

The catalog number for P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL is BNC040669-500.

What sizes are available for P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL?

P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL in stock?

P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL?

P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL?

Estimated delivery time for P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL?

The safety data sheet for P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd0299061270361e10107e/msds/bnc040669-500.pdf.

What is P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL used for?

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.