P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL — image 1 of 4

Product details

P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL

Catalog number BNC680669-100

Price for BNC680669-100

Size / count 1x 100 µL

About this product

This MAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-betanduced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC680669-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CDKN1B
Gene ID: 1027
NCBI Gene ID: 238990
UniProt: P46527
Swiss Prot: P46527
Host: Mouse
Species Reactivity: Human, Monkey, Mouse, Rat
Reactivity: KIP1|p27
Immunogen: Purified GST-p27 fusion protein of human origin
Target Antigen: KIP1 | p27
Clonality: Monoclonal
Clone: SX53G8
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE|Flow, intracellular (verified) |IF (verified) |IP (published) |WB (published)
Field of Research: Cell cycle, Tumor suppressors
Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 25-26 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications._x000D_ 1. PNAS USA (1997) 94:6380-6385. (western; immunoprecipitation; IHC, FFPE)_x000D_ 2. Blood (2005) 105(9): 3691. (western)_x000D_ 3. Anticancer Res (2017) 37: 2407-2415. (IHC, FFPE; IF, western)_x000D_
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF568

Frequently Asked Questions

What is the catalog number for P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL?

The catalog number for P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL is BNC680669-100.

What sizes are available for P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL?

P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL in stock?

P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL?

P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL?

Estimated delivery time for P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL?

The safety data sheet for P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02ac061270361e107e4e/msds/bnc680669-100.pdf.

What is P27 / KIP1 (SX53G8), CF568 conjugate, 0.1mg/mL used for?

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.