P27 / KIP1 (SX53G8), CF640R conjugate, 0.1mg/mL

About this product

This MAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-betanduced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC400669-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CDKN1B

Gene ID

1027

NCBI Gene ID

238990

UniProt

P46527

Swiss Prot

P46527

Host

Mouse

Species Reactivity

Human, Monkey, Mouse, Rat

Reactivity

KIP1|p27

Immunogen

Purified GST-p27 fusion protein of human origin

Target Antigen

KIP1 | p27

Clonality

Monoclonal

Clone

SX53G8

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE|Flow, intracellular (verified) |IF (verified) |IP (published) |WB (published)

Field of Research

Cell cycle, Tumor suppressors

Positive Control

ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

25-26 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications._x000D_ 1. PNAS USA (1997) 94:6380-6385. (western; immunoprecipitation; IHC, FFPE)_x000D_ 2. Blood (2005) 105(9): 3691. (western)_x000D_ 3. Anticancer Res (2017) 37: 2407-2415. (IHC, FFPE; IF, western)_x000D_

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF640R