P53 (PAb122), CF647 conjugate, 0.1mg/mL

About this product

Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min) . p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. PAb122 binds to the C-terminus (aa370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb122 blocked re-entry into the S-phase of the cell cycle. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470338-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TP53

Gene ID

7157

NCBI Gene ID

654481

UniProt

P04637

Swiss Prot

P04637

Host

Mouse

Species Reactivity

Dog, Hamster, Human, Monkey, Mouse, Rat

Reactivity

P53 Tumor Suppressor Protein

Immunogen

SV40-transformed mouse B4 cells

Target Antigen

p53 Tumor Suppressor Protein

Clonality

Monoclonal

Clone

PAb122

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published) |IF (published) |IP (published) |WB (published)

Field of Research

Apoptosis, Tumor suppressors

Positive Control

MDA-MB-231 or A431 Cells. Breast or Colon carcinoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

53 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Mol Cell Biol (1985) 5(7): 1601-1610. (IP) Mol Cell Biol (1990) 10(11): 5772-5781. (IP, IF, RIA) Oncogene (1991) 6:1499-1506. (Flow, IF, IP) BBRC (2009) 387: 772-777. (WB, IF)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647