Mouse COL1A1 Protein, His Tag, low endotoxin (MALS verified)

About this product

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1 (I) chains and one alpha 2 (I) chain, although homotrimers consisting of three identical alpha 1 (I) chains have also been described .

Specifications

Catalog number

CO1-M5243-1mg

Size / count

1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1 (I) chains and one alpha 2 (I) chain, although homotrimers consisting of three identical alpha 1 (I) chains have also been described .

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 28.8 kDa. The protein migrates as 33-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P11087

Expression Region

Leu 1218 - Val 1453

Host

HEK293

Target

COL1A1

Conjugation

Unconjugated

Tag

N-10xHis

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

90%

Format

Powder

Buffer

50 mM Tris, 150 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

28.8 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

1mg*1

Host or Source

HEK293

Species

Mouse

Protein ID

P11087

Preservative

Trehalose