Phosphotyrosine (PY20), 0.2mg/mL

About this product

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0232-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

P-Tyr

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

UniProt

Not Applicable

Swiss Prot

Not Applicable

Host

Mouse

Species Reactivity

Pan species

Reactivity

Phosphotyrosine

Immunogen

Phosphotyrosine conjugated to KLH

Target Antigen

Phosphotyrosine

Clonality

Monoclonal

Clone

PY20

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |ELISA (published) |IHC, FFPE (verified) |IP (published) |WB (published)

Field of Research

Signal transduction

Positive Control

MCF-7, MDA-231, T47-D cells or breast carcinoma

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

Depends upon the phosphorylated target

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1983) 264(34): 20163-20166. (IP) J Immunological Meth (1988) 109: 277-285. (ELISA) Mol Endocrinol (1998) 12(4): 536-543. (WB) Fert Stert (1999) 71(5): P919-923. (IF)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA