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Product details

Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL

Catalog number BNC040232-500

Price for BNC040232-500

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Size / count 1x 500 µL

About this product

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC040232-500
Size / count
1x 500 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
P-Tyr
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
UniProt
Not Applicable
Swiss Prot
Not Applicable
Host
Mouse
Species Reactivity
Pan species
Reactivity
Phosphotyrosine
Immunogen
Phosphotyrosine conjugated to KLH
Target Antigen
Phosphotyrosine
Clonality
Monoclonal
Clone
PY20
Type
Primary
Source
Animal
Origin
Animal
Applications
IF (published) |ELISA (published) |IHC, FFPE (verified) |IP (published) |WB (published)
Field of Research
Signal transduction
Positive Control
MCF-7, MDA-231, T47-D cells or breast carcinoma
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
Depends upon the phosphorylated target
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1983) 264(34): 20163-20166. (IP) J Immunological Meth (1988) 109: 277-285. (ELISA) Mol Endocrinol (1998) 12(4): 536-543. (WB) Fert Stert (1999) 71(5): P919-923. (IF)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2b
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF405S

Frequently Asked Questions

What is the catalog number for Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL?

The catalog number for Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL is BNC040232-500.

What sizes are available for Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL?

Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL in stock?

Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL?

Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL?

Estimated delivery time for Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL?

The safety data sheet for Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Phosphotyrosine (PY20), CF405S conjugate, 0.1mg/mL used for?

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

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