Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL

Catalog number BNC470232-500

Price for BNC470232-500

Size / count 1x 500 µL

About this product

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC470232-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: P-Tyr
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
UniProt: Not Applicable
Swiss Prot: Not Applicable
Host: Mouse
Species Reactivity: Pan species
Reactivity: Phosphotyrosine
Immunogen: Phosphotyrosine conjugated to KLH
Target Antigen: Phosphotyrosine
Clonality: Monoclonal
Clone: PY20
Type: Primary
Source: Animal
Origin: Animal
Applications: IF (published) |ELISA (published) |IHC, FFPE (verified) |IP (published) |WB (published)
Field of Research: Signal transduction
Positive Control: MCF-7, MDA-231, T47-D cells or breast carcinoma
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: Depends upon the phosphorylated target
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Chem (1983) 264(34): 20163-20166. (IP) J Immunological Meth (1988) 109: 277-285. (ELISA) Mol Endocrinol (1998) 12(4): 536-543. (WB) Fert Stert (1999) 71(5): P919-923. (IF)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2b
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF647

Frequently Asked Questions

What is the catalog number for Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL?

The catalog number for Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL is BNC470232-500.

What sizes are available for Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL?

Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL in stock?

Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL?

Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL?

Estimated delivery time for Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL?

The safety data sheet for Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02b8061270361e10c3f5/msds/bnc470232-500.pdf.

What is Phosphotyrosine (PY20), CF647 conjugate, 0.1mg/mL used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.