Pmel17 / gp100 / SILV (HMB45), 0.2mg/mL

About this product

By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, malignant melanoma, and angiomyolipoma (PEComa) .Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0444-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

PMEL

Gene ID

6490

NCBI Gene ID

95972

UniProt

P40967

Swiss Prot

P40967

Host

Mouse

Species Reactivity

Human

Reactivity

Gp100|Pmel17|SILV

Immunogen

Extract of pigmented melanoma metastases from lymph nodes

Target Antigen

gp100 | Pmel17 | SILV

Clonality

Monoclonal

Clone

HMB45

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified) |WB (verified)

Field of Research

Cancer

Positive Control

SK-MEL-28 cells or Melanoma

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

90-100 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA