Pmel17 / gp100 / SILV (NKI-beteb), CF647 conjugate, 0.1mg/mL

About this product

By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470226-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

PMEL

Gene ID

6490

NCBI Gene ID

95972

UniProt

P40967

Swiss Prot

P40967

Host

Mouse

Species Reactivity

Horse, Human

Reactivity

Gp100|Pmel17|SILV

Immunogen

Membranes from a human melanoma metastasis

Target Antigen

gp100 | Pmel17 | SILV

Clonality

Monoclonal

Clone

NKI-beteb

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published) |IF (published) |IHC, frozen (published) |ELISA (published) |IHC, FFPE (verified) |IP (published)

Field of Research

Cancer

Positive Control

SK-MEL-28 cells or Melanoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

90-100 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Am J Pathol (1988) 130(1): 179. (mAb characterization; IHC, FFPE; IF; ELISA; IP; WB) Am J Pathol (1993) 143(6): 1579. (IHC, frozen; IF) J Biol Chem (2004) 279(27): 28330-28338. (epitope mapping; IP; reports that NKI-beteb does not work well for WB) Exp Dermatol (2010) 19(8): e282-e285. (IF; Flow, intracellular)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647