Smooth Muscle Actin (1A4), CF640R conjugate, 0.1mg/mL

About this product

Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC400665-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

ACTA2|Actin Alpha 2 Smooth Muscle Aorta|Actin Alpha Smooth Muscle|Actin Aortic Smooth Muscle|Actin Vascular Smooth Muscle|ACTSA|ACTVS|Alpha 2 Actin|Alpha Cardiac Actin|Alpha-actin-2|ASMA|Cell Growth-inhibiting Gene 46 Protein|Growth Inhibiting Gene 46

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

ACTA2

Gene ID

59

NCBI Gene ID

500483

UniProt

P62736

Swiss Prot

P62736

Host

Mouse

Species Reactivity

Baboon, Cat, Chicken, Cow, Dog, Goat, Guinea pig, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep

Reactivity

Smooth Muscle Actin

Immunogen

N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.

Target Antigen

Smooth Muscle Actin

Clonality

Monoclonal

Clone

1A4

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |IF (verified) |IHC, FFPE (verified)

Field of Research

Cytoskeleton

Positive Control

Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

42 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF640R