Recombinant Human CD16b/FCGR3B Protein

Specifications

Catalog number

PKSH031290

Size / count

1 mg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Carbohydrate sulfotransferase 15; also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST; B-cell RAG-associated gene protein; CHST15 and BRAG; is a single-pass type II membrane protein which belongs to thesulfotransferase 1 family. CHST15 / BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen; lymph node; tonsil; bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B; pre-B; and mature B-cell lines; it colocalizes with RAG1. CHST15 / BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4;6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence; GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4); to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 / BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression.

CAS Number

9000-83-3

Synonyms

CD16b and FCGR4B, FCG3, FCGR3, FCRIII, FCRIIIb, Fc-gamma RIII-beta, FcR-10, IgG Fc receptor III-1, Low affinity immunoglobulin gamma Fc region receptor III-B

UniProt

O75015

Accession Number

O75015-1

Host

E. coli

Origin Species

Human

Tag

None

Sequence

Met 18-Gly 193

Applications

BLI

Field of Research

Immunology

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Using the Octet RED System, the affinity constant (Kd) of CD16b bound to Human IgG1 was 80nM.

Reconstitution

Please refer to the printed manual for detailed information.

Molecular Weight

22 kDa

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.