TAG-72 / CA72.4 (B72.3), CF647 conjugate, 0.1mg/mL

About this product

Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470276-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CA72.4, Tumor associated glycoprotein 72

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

tag-72

Gene ID

182875

NCBI Gene ID

Not Known

UniProt

Not Known

Swiss Prot

Not Known

Host

Mouse

Species Reactivity

Cow, Dog, Hamster, Human, Rat

Reactivity

CA72.4|TAG-72

Immunogen

Membrane-enriched fraction of a human breast carcinoma liver metastasis

Target Antigen

CA72.4 | TAG-72

Clonality

Monoclonal

Clone

B72.3

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |IHC, FFPE (verified) |IP (published) |RIA (published) |WB (published)

Field of Research

Cancer

Positive Control

Jurkat cells, Breast or lung carcinoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

220 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1981) 78(5): 3199-3203. (IHC, FFPE; RIA) Clin Exp Metastasis (2012) 29:229-238. (Flow, surface; IP; WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647