Testosterone (4E1G2), CF647 conjugate, 0.1mg/mL

About this product

This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~1010 M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone <0.1%, 5-alpha DHT 0.8%, estradiol <0.1% and progesterone <0.1%. Testosterone, a steroid hormone from the androgen group, is derived from cholesterol. Testosterone is primarily secreted in the testes, but the ovaries and adrenal glands also produce smaller amounts. It is the principal male sex hormone and is responsible for the maturation of sex organs and the development of male secondary sex characteristics. The hormone is also involved in the growth of muscle mass and increases bone density and maturation. It functions by activating the androgen receptor and converting to estradiol and activation of estrogen receptors. Testosterone has been employed therapeutically to treat many conditions including infertility, lack of libido, osteoporosis, height growth and bone marrow stimulation. Anti-testosterone antibody may also prove useful in identification of testicular tumors arising from interstitial cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470250-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

17Î2-Hydroxy-3-oxo-4-androstene, 17Î2-Hydroxy-4-androsten-3-one, 4-Androsten-17Î2-ol-3-one, trans-Testosterone

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

UniProt

Not Applicable

Swiss Prot

Not Applicable

Host

Mouse

Species Reactivity

Pan species

Reactivity

Testosterone

Immunogen

Testosterone 3 CMO conjugated to BSA

Target Antigen

Testosterone

Clonality

Monoclonal

Clone

4E1G2

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified) |RIA (published)

Field of Research

Endocrinology

Positive Control

Interstitial or Leydid cells in testis.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

288.42Da

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Hum Reprod Sci (2018) 11(3): 291-296. (RIA) Syst Biol Reprod Med (2018) 64(3): 191-201. (RIA)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647