HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL

Catalog number BNC741574-100

Price for BNC741574-100

Size / count 1x 100 µL

About this product

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC741574-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78) ; Member of PAS superfamily 1 (MOP1) ; PAS domain-containing protein 8 (PASD8)
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: HIF1A
Gene ID: 3091
NCBI Gene ID: 597216
UniProt: Q16665
Swiss Prot: Q16665
Host: Mouse
Species Reactivity: Cow, Dog, Human, Mouse, Rat
Reactivity: HIF1 Alpha
Immunogen: GST-human HIF-1A amino acids 329-530 fusion protein
Target Antigen: HIF1 Alpha
Clonality: Monoclonal
Clone: ESEE122
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (published) |WB (published)
Positive Control: Cobalt chloride treated HeLa cells. Colon, Renal, Breast or Bladder Carcinomas.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% rBSA, 0.05% azide
Molecular Weight: 92-110 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer (2006) 107(4): 757-766. (IHC, FFPE) Biol Pharm Bull (2017) 40(1): 82-87. (WB)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), Bos taurus (bovine)
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF740

Frequently Asked Questions

What is the catalog number for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL?

The catalog number for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL is BNC741574-100.

What sizes are available for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL?

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL in stock?

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL?

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL?

The safety data sheet for HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd0297061270361e100549/msds/bnc741574-100.pdf.

What is HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF740 conjugate, 0.1mg/mL used for?

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.