TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL

Catalog number BNC940994-100

Price for BNC940994-100

Size / count 1x 100 µL

About this product

This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC940994-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: APC1; Cachectin; Differentiation inducing factor (DIF) ; Macrophage cytotoxic factor (MCF) ; Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: TNF
Gene ID: 7124
NCBI Gene ID: 241570
UniProt: P01375
Swiss Prot: P01375
Host: Mouse
Species Reactivity: Human
Reactivity: TNF-Alpha
Immunogen: Recombinant human TNF-alpha
Target Antigen: TNF-Alpha
Clonality: Monoclonal
Clone: J2D10
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (published) |ELISA (published)
Field of Research: Cytokines, Immunology
Positive Control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 17 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Exp Med (1992) 175: 81-90. (ELISA) FASEB J (2002) 16(13): 1-19. (IHC, FFPE)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL?

The catalog number for TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL is BNC940994-100.

What sizes are available for TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL?

TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL in stock?

TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL?

TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL?

The safety data sheet for TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is TNF alpha (J2D10), CF594 conjugate, 0.1mg/mL used for?

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.