TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL

Catalog number BNC680656-500

Price for BNC680656-500

Size / count 1x 500 µL

About this product

This MAb recognizes human 17-26 kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha) . TNF-alpha can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC680656-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: APC1; Cachectin; Differentiation inducing factor (DIF) ; Macrophage cytotoxic factor (MCF) ; Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: TNF
Gene ID: 7124
NCBI Gene ID: 241570
UniProt: P01375
Swiss Prot: P01375
Host: Mouse
Species Reactivity: Human
Reactivity: TNF-Alpha
Immunogen: Recombinant human TNF-alpha
Target Antigen: TNF-Alpha
Clonality: Monoclonal
Clone: TNF656
Type: Primary
Source: Animal
Origin: Animal
Field of Research: Cytokines, Immunology
Positive Control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 17 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF568

Frequently Asked Questions

What is the catalog number for TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL?

The catalog number for TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL is BNC680656-500.

What sizes are available for TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL?

TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL in stock?

TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL?

TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL?

Estimated delivery time for TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL?

The safety data sheet for TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02a2061270361e1048ab/msds/bnc680656-500.pdf.

What is TNF alpha (TNF656), CF568 conjugate, 0.1mg/mL used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.