SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag

Catalog number SPD-C52Hf-1mg

Discontinued

Price for SPD-C52Hf-1mg

Size / count 1 mg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 S protein RBD (F490L), His Tag (SPD-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (F490L)).

Specifications

Catalog number: SPD-C52Hf-1mg
Size / count: 1 mg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 26.6 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag?

The catalog number for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is SPD-C52Hf-1mg.

Is SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag in stock?

SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag?

SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag?

Estimated delivery time for SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag used for?

SARS-CoV-2 S protein RBD (F490L), His Tag (SPD-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (F490L)).

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.