Transglutaminase II (TGM2/419), CF647 conjugate, 0.1mg/mL

About this product

Recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II) . Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470419-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Protein-glutamine gamma-glutamyltransferase 2; TG (C) ; TG2; TGase C; TGase H; TGase-2; TgaseII; TGC; TGM2; Tissue type transglutaminase; Transglutaminase 2 C polypeptide; Transglutaminase H; tTG; tTGas

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

TGM2

Gene ID

7052

NCBI Gene ID

517033

UniProt

P21980

Swiss Prot

P21980

Host

Mouse

Species Reactivity

Human, Monkey, Mouse, Rabbit, Rat

Reactivity

Transglutaminase-II

Immunogen

Recombinant full-length human TGM2 protein

Target Antigen

Transglutaminase-II

Clonality

Monoclonal

Clone

TGM2/419

Type

Primary

Source

Animal

Origin

Animal

Field of Research

Cytoskeleton

Positive Control

HUVEC Cells. Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin (e.g. intestine)

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

77-85 kDa

References & Citations

Yamanishi, K., et al. 1991. Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture. Biochem. Biophys. Res. Commun. 175: 906-913. |

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647